A Protocol for Conventional Sleep Deprivation Methods in Rats

  • Javad Mahmoudi
  • Nahid Ahmadian neuroscience research center
  • Fereshteh Farajdokht
  • Alireza Majdi
  • Marjan Erfani


Several experimental methods have been developed to restrict the sleep in laboratory animals. Among them, the flowerpot and Modified Multiple Platform (Modified-MP) techniques are widely used largely due to their simplicity and low cost. However, better application of these techniques may need some technical points to be considered. Given this, the present guide besides focusing on the critical points of these techniques gives a brief instruction for setting up and executing them.

Short Communication